Studying cells in 3D could reveal new cancer targets

Baltimore 21 June 2010Showing movies in 3D has produced a box-office bonanza in recent months. Could viewing cell behaviour in three dimensions lead to important advances in cancer research? A new study led by Johns Hopkins University engineers indicates it may happen. Looking at cells in 3D, the team members concluded, yields more accurate information that could help develop drugs to prevent cancer's spread. The study, a collaboration with researchers at Washington University in St. Louis, appears in the June issue of Nature Cell Biology.

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"Finding out how cells move and stick to surfaces is critical to our understanding of cancer and other diseases. But most of what we know about these behaviours has been learned in the 2D environment of Petri dishes", stated Denis Wirtz, director of the Johns Hopkins Engineering in Oncology Center and principal investigator of the study. "Our study demonstrates for the first time that the way cells move inside a three-dimensional environment, such as the human body, is fundamentally different from the behaviour we've seen in conventional flat lab dishes. It's both qualitatively and quantitatively different."

One implication of this discovery is that the results produced by a common high-speed method of screening drugs to prevent cell migration on flat substrates are, at best, misleading, said Denis Wirtz, who also is the Theophilus H. Smoot Professor of Chemical and Biomolecular Engineering at Johns Hopkins. This is important because cell movement is related to the spread of cancer, Denis Wirtz said. "Our study identified possible targets to dramatically slow down cell invasion in a three-dimensional matrix."

When cells are grown in two dimensions, Denis Wirtz said, certain proteins help to form long-lived attachments called focal adhesions on surfaces. Under these 2D conditions, these adhesions can last several seconds to several minutes. The cell also develops a broad, fan-shaped protrusion called a lamella along its leading edges, which helps move it forward. "In 3D, the shape is completely different", Denis Wirtz stated. "It is more spindlelike with two pointed protrusions at opposite ends. Focal adhesions, if they exist at all, are so tiny and so short-lived they cannot be resolved with microscopy."

The study's lead author, Stephanie Fraley, a Johns Hopkins doctoral student in Chemical and Biomolecular Engineering, said that the shape and mode of movement for cells in 2D are merely an "artifact of their environment", which could produce misleading results when testing the effect of different drugs. "It is much more difficult to do 3D cell culture than it is to do 2D cell culture", Stephanie Fraley stated. "Typically, any kind of drug study that you do is conducted in 2D cell cultures before it is carried over into animal models. Sometimes, drug study results don't resemble the outcomes of clinical studies. This may be one of the keys to understanding why things don't always match up."

Stephanie Fraley's faculty supervisor, Denis Wirtz, suggested that part of the reason for the disconnection could be that even in studies that are called 3D, the top of the cells are still located above the matrix. "Most of the work has been for cells only partially embedded in a matrix, which we call 2,5-D", he stated. "Our paper shows the fundamental difference between 3D and 2,5-D: Focal adhesions disappear, and the role of focal adhesion proteins in regulating cell motility becomes different."

Denis Wirtz added that "because loss of adhesion and enhanced cell movement are hallmarks of cancer", his team's findings should radically alter the way cells are cultured for drug studies. For example, the team found that in a 3D environment, cells possessing the protein zyxin would move in a random way, exploring their local environment. But when the gene for zyxin was disabled, the cells traveled in a rapid and persistent almost one-dimensional pathway far from their place of origin.

Stephanie Fraley said such cells might even travel back down the same pathways they had already explored. "It turns out that zyxin is misregulated in many cancers", Stephanie Fraley stated. Therefore, she added, an understanding of the function of proteins like zyxin in a 3D cell culture is critical to understanding how cancer spreads, or metastasizes. "Of course tumour growth is important, but what kills most cancer patients is metastasis", she stated.

To study cells in 3D, the team coated a glass slide with layers of collagen-enriched gel several millimeters thick. Collagen, the most abundant protein in the body, forms a network in the gel of cross-linked fibers similar to the natural extracellular matrix scaffold upon which cells grow in the body. The researchers then mixed cells into the gel before it set. Next, they used an inverted confocal microscope to view from below the cells traveling within the gel matrix. The displacement of tiny beads embedded in the gel was used to show movement of the collagen fibers as the cells extended protrusions in both directions and then pulled inward before releasing one fiber and propelling themselves forward.

Stephanie Fraley compared the movement of the cells to a person trying to maneuver through an obstacle course crisscrossed with bungee cords. "Cells move by extending one protrusion forward and another backward, contracting inward, and then releasing one of the contacts before releasing the other", she stated. Ultimately, the cell moves in the direction of the contact released last.

When a cell moves along on a 2D surface, the underside of the cell is in constant contact with a surface, where it can form many large and long-lasting focal adhesions. Cells moving in 3D environments, however, only make brief contacts with the network of collagen fibers surrounding them - contacts too small to see and too short-lived to even measure, the researchers observed.

"We think the same focal adhesion proteins identified in 2D situations play a role in 3D motility, but their role in 3D is completely different and unknown", Denis Wirtz stated. "There is more we need to discover."

Stephanie Fraley said her future research will be focused specifically on the role of mechanosensory proteins like zyxin on motility, as well as how factors such as gel matrix pore size and stiffness affect cell migration in 3D.

Co-investigators on this research from Washington University in St. Louis were Gregory D. Longmore, a professor of medicine, and his postdoctoral fellow Yunfeng Feng, both of whom are affiliated with the university's BRIGHT Institute. Gregory D. Longmore and Denis Wirtz lead one of three core projects that are the focus of the Johns Hopkins Engineering in Oncology Center, a National Cancer Institute-funded Physical Sciences in Oncology Center. Additional Johns Hopkins authors, all from the Department of Chemical and Biomolecular Engineering, were Alfredo Celedon, a recent doctoral recipient; Ranjini Krishnamurthy, a recent bachelor's degree recipient; and Dong-Hwee Kim, a current doctoral student.

Funding for the research was provided by the National Cancer Institute.


Source: Johns Hopkins University

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